European Journal of Experimental Biology Libre accès

Abstrait

Molecular melting profile of MDR1 gene in doxorubicin and docetaxel treated MCF-7 and natural MCF-7 cell line

Trebunova Marianna, Laputkova Galina, Lacjakova Kamila, Verebova Aneta, Imrich Geci and Sabo Jan

In this study we compared nucleotide and amino acid composition of MDR1 gene in natural MCF-7 cell line (MCF- 7/wt) and in MCF-7 cell line treated with combination of cytostatics doxorubicin (DOX) and docetaxel (DOC) (MCF-7/adr). MCF-7 cell line was exposed to cytostatics for six hours. The concentration of cytostatics was 5μg/ml for DOC and 0.15μg/ml for DOX. We wanted to determine some changes of cell viability as well as some changes at the molecular level. For this purpose we performed cell proliferation assay and HRM analysis of purified DNA. We obtained results using the evaluation programs BioEdit Sequence Alignment Editor. We observed the change of cell viability and also some changes in percentage of nucleotides and amino acid of MDR1 gene. We found out whether application of 5μg/ml for DOC and 0.15ug/ml for DOX on MCF-7 cell line for six hours retarded metabolic activity to 96.98% (p<0.01). We determined the change in molecular profile of MCF-7. In MCF-7/adr, the number of amino acids was 661 in comparison with MCF-7/wt, where the number of amino acids was 646.