Gaurav Jain and Ameeta Argal
Triticum aestivum has been traditionally known to have several medicinal properties to treat various disorders. It has been used as a general purpose health tonic for several years. It is used to treat a number of conditions including the thalessmia, hypolipidemic, hypoglycemic, ulcers, cleansing, fissures, injuries, cuts and wounds. In folk medicine, practitioners used wheatgrass for the treatment of cystitis, gout, rheumatic pain, chronic skin disorders and constipation. The present study was carried out to investigate total phenolic, total flavonoid content and estimation of quercetin by high performance liquid chromatography in methanolic extract of Triticum aestivum ninth day grass. Total phenolic content was determined by using the folin ciocalteu assay. The content of total flavonoids was also measured spectrophotometrically by using aluminum chloride colorimetric assay. Total phenolic content and total flavonoid content analysis was performed using methanol as solvent, gallic acid and rutin were used for standard curve respectively. High performance liquid chromatography was used for the estimation of quercetin in the extract. It is a powerful analytical technique because of its reliability, simplicity, reproducibility and speed. The purpose of the work is to investigate the chemical constituents responsible for the role of wheatgrass which has been used in traditional medicine to improve healthcare.
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