Deepak R Chandra and Thoyajaksha Sakaleshpur
Numerous scientific studies showed that tissue culture induced mutation has been detected in all species studied. Therefore, it is very important to study the degree of genetic similarity by comparing the gene sequence of regenerated plants with the mother plant. In this study, a Polymorphic Chain Reaction (PCR) based methods such as RAPD and ISSR marker techniques were used to study the genetic similarity of in-vitro cultured plants of Ventilago maderaspatana Geartn. Fourteen individuals were randomly tagged from the population of regenerated plants for the genetic similarity analysis. ISSR method carried using 22 primers from which a total of 98 clear and reproducible bands was scored. Out of 98 bands, 72 were monomorphic bands (73.47%) and 26 were polymorphic bands (26.53%). Dendrogram cluster analysis of data generated from 22 ISSR primers showed an estimated genetic distance of 0.07. RAPD method carried using 21 primers from which a total of 194 clear and reproducible bands was scored. Out of 194 bands, 126 were monomorphic bands (64.94%) and 68 were polymorphic bands (35.05%). Dendrogram cluster analysis of data generated from 21 RAPD primers showed an estimated genetic distance of 0.065. ISSR and RAPD data analyzed using NTSYS-pc showed that micro propagated plants exhibit maximum similarity with the mother plant.
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