European Journal of Experimental Biology Libre accès

Abstrait

Detection of Lactococcus garvieae and Vibrio cholerae in some aquatic fishes of Persian Gulf using polymerase chain reaction

Faham Khamesipour, Masoud Shahrani, Abed Raki and Abbas Doosti

Lactococcus garvieae (L. garvieae) and Vibrio cholerae (V. cholerae) causes emerging zoonotic disease in all over the world. In this research, we describe the lactococcosis and Vibrio cholerae in some aquatic fishes of Persian Gulf using Polymerase Chain Reaction (PCR) method during November and December 2013 in Iran. L. garvieae infections were detected in 0.00%, 0.00%, 10%, 10%,10%, 10%, 20% and 20% of the sampled Stromateidae (n=20), Pleuronectiformes (n=20), Scomberomorus commerson (n=20), Oyster (Pinctada Pearl) (n=20), Shrimp (n=30), Sawfishes (Pristis zijsron) (n=20), and Crab (Brachyura) (n=20) respectively. Also, V. cholerae infections were detected in 0.00%, 0.00%, 0.00%, 10%,13%, 0.00% and 30% of the sampled Stromateidae (n=20), Pleuronectiformes (n=20), Scomberomorus commerson (n=20), Oyster (Pinctada Pearl) (n=20), Shrimp (n=30), Sawfishes (Pristis zijsron) (n=20), and Crab (Brachyura) (n=20) respectively. In the present study, we succeeded in detecting and identifying two major species of lactococcosis and vibriosis disease by PCR technique in some aquatic fishes of Persian Gulf of Iran. Further attempts for molecular identification of these strains and introduction of new species are however needed.