Revue de biochimie et de biologie moléculaire Libre accès

Abstrait

Computational Approach to Study Role of Active Site Amino Acids of Alpha- Amylases in Thermostability of Pyrococcus Furiosus and Bacillus sp.

Tayyaba Huma1*, Ghulam Mustafa2, Ayesha sethi3, Arooma Maryam4, Muhammad Ali5

Alpha amylase has a wide range of applications in starch processing, textile industry and its role as a detergent is also well defined. In this study rational approach of protein engineering to analyse the nature of the active site of thermostable alpha amylases via in silico computational tools was excercised. Sequential and structural analysis of alpha amylases from Pyrococcus furiosus (100ºC-105ºC), Bacillus licheniformis (90-95ºC) and Bacillus amyloliquefaciens (72ºC) was carried out.