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Column Chromatography Purified Phytochemicals Identified in Phragmytes vallatoria Leaf Ethanolic Extract

Naga Vamsi Krishna. A, Praveen Kumar .P, Mahendran. B, Venkata Raman. B

Phragmytes vallatoria belongs to the family poaceae. The complexity of leaf ethanolic extract can be simplified through column chromatography. Different solvent mixtures were used in elution systems i.e., hexane, ethyl acetate and methanol. The concentrated ethanolic leaf extract of 100 g was fractionated by column chromatography on silica gel (60-120 mesh). Major fractions were (hexane + ethyl acetate 9:1 and ethyl acetate + methanol 8.5: 1.5 fraction) and identified the major phytochemicals through NIST Electron Ionization Mass database. The identified compounds were 9, 12, 15-Octadecatrienoic acid, ethyl ester, (Z, Z, Z)-, Androstane-11, 17-dione, 3-hydroxy-, (3.alpha. 5. alpha)-, 9,12-Octadecadienoic acid ethyl ester and 9-12-15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)-. Only one peak was observed in ethyl acetate and methanol (8.5: 1.5) fraction and it was subjected to GC-MS and HPLC. The structures were (Benzenamine, 3-(methylthio)-, Morpholine, Phenanthrene and 3-Eicosene, (E).