Ratnasri P.V and Hemalatha K.P.J
The synthesis of nanoparticles from the microbes is a boon for advance research in nanotechnology. In this study, silver nanoparticles were synthesized using the fungus Aspergillus fumigatus with an aqueous solution of AgNO3. Synthesized silver nanoparticles (Ag-NPs) were characterized through UV-visible spectrophotometer and Fourier Transform Infrared Spectroscopy (FTIR). Maximum absorbance was observed at 420 nm in visible region. The nature of coordination between bioactive compounds secreted by fungi and silver ions were analyzed through FTIR spectroscopy. The reduction of silver ions was due to amino groups of proteins and other functional groups in the cell free filtrate of fungi. The reduction of silver ions leads to the formation of stable protein capped silver nanoparticles. The Ag-NPs and Ag-NPs + Chloramphenicol (Ab) possess potential antimicrobial activity against to Escherichia coli, Klebsiella pneumonia, Bacillus cereus, Staphylococcus aureus and Streptococcus sp.